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Bench Scientist

Job Post Index 20602E-Sy##
Location Cambridge, MA
Description: Description
  • Responsible for screening and VH-VL molecular cloning of antibodies from hybridomas generated to support biotherapeutic target validation efforts.
  • Work in close coordination with scientists to screen sera from immunized rodents, generate and screen hybridomas and complete VH-VL molecular cloning of prioritized hybridoma panels.
  • Implement ELISA, flow cytometry and hybridoma SOP’s to generate novel antibodies
  • Track project timelines
  • Prepare and deliver detailed presentations, both written and verbal, to project teams
Skills: Qualifications
  • Experience with screening of immune sera by ELISA and flow cytometry
  • Experience with Fusions and screening of hybridomas
  • Experience with VH-VL molecular cloning of antibodies from hybridomas
  • Experience with transient expression of antibody clones, including cells employed, methods and molecular design of expression vectors.
  • Experience with screening of sera by ELISA and flow cytometry
  • Experience with VH-VL molecular cloning from hybridomas and primary B cells
  • Consistently accurate performance of hands-on tasks and detailed, accurate reporting of outcomes
  • Effective communication and coordination of work in highly matrixed project team environment

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